Lysis Buffer 6 (1 x 21 mL) 895561 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemLysis Buffer 6 (1 x 21 mL)
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog Number895561
Quantity1 Each
ApplicationsELISA
Brand/TrademarkR&D Systems, a Bio-Techne Brand
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) A small-molecule PI3Kα activator for cardioprotection and neuroregenerationNatureJune 1, 2023Gong GQ, Bilanges B, Allsop B, Masson GR, Roberton V, Askwith T, Oxenford S, Madsen RR, Conduit SE, Bellini D, et al.min at 37°C, 5% CO2 before being washed with ice-cold PBS and lysed in lysis buffer 6 (R&D Systems #895561) and freezing at -80°C. Levels of pAKTS473 were determined using the phospho-AKT (S473) pan-specific

(2) Resistance to tyrosine kinase inhibitors promotes renal cancer progression through MCPIP1 tumor-suppressor downregulation and c-Met activationCell death & diseaseSeptember 22, 2022Marona P, Górka J, Kwapisz O, Jura J, Rys J, Hoffman RM, Miekus K.

(3) PI3K/Akt pathway mediates the positive inotropic effects of insulin in Langendorff-perfused rat heartsScientific reportsJune 13, 2022Nakadate Y, Kawakami A, Sato H, Sato T, Oguchi T, Omiya K, Matsuoka T, Schricker T, Matsukawa T.

(4) Degradation and inhibition of epigenetic regulatory protein BRD4 exacerbate Alzheimer's disease-related neuropathology in cell modelsThe Journal of biological chemistryApril 1, 2022Zhang S, Bai P, Lei D, Liang Y, Zhen S, Bakiasi G, Pang H, Choi SH, Wang C, Tanzi RE, et al.

(5) Genome wide CRISPR/Cas9 screen identifies the coagulation factor IX (F9) as a regulator of senescenceCell death & diseaseFebruary 19, 2022Carpintero-Fernández P, Borghesan M, Eleftheriadou O, Pan-Castillo B, Fafián-Labora JA, Mitchell TP, Yuste A, Ogrunc M, Nightingale TD, Mayan M, et al.

(6) PARP1 Inhibitor and Trabectedin Combination Does Not Increase Tumor Mutational Burden in Advanced Sarcomas-A Preclinical and Translational StudyCancersDecember 15, 2021Pignochino Y, Crisafulli G, Giordano G, Merlini A, Berrino E, Centomo ML, Chiabotto G, Brusco S, Basiricò M, Maldi E, et al.

(7) EphA2 Expression in Bone Sarcomas: Bioinformatic Analyses and Preclinical Characterization in Patient-Derived Models of Osteosarcoma, Ewing's Sarcoma and ChondrosarcomaCellsOctober 26, 2021Giordano G, Merlini A, Ferrero G, Mesiano G, Fiorino E, Brusco S, Centomo ML, Leuci V, D'Ambrosio L, Aglietta M, et al.

(8) Androgen Receptor Activation in Glioblastoma Can Be Achieved by Ligand-Independent Signaling through EGFR-A Potential Therapeutic TargetInternational journal of molecular sciencesOctober 11, 2021Zalcman N, Gutreiman M, Shahar T, Weller M, Lavon I.plugin in Fiji [46]. For Western blotting and Elisa assay, the cells were lysed with Buffer 6 (cat. no. 895561, R&D Systems, Minneapolis, MN, USA,) supplemented with protease and phosphatase inhibitor (ab201120;

(9) [18F]-FDHT PET/CT as a tool for imaging androgen receptor expression in high-grade gliomaNeuro-Oncology AdvancesMarch 19, 2021Marina Orevi, Ofer Shamni, Nomi Zalcman, Alexandre Chicheportiche, Anat Mordechai, Samuel Moscovici, Yigal Shoshan, Tal Shahar, Hanna Charbit, Mijal Gutreiman, et al.minor modifications. Briefly, tissue samples were homogenized in 200 µL of Lysis Buffer 6# (catalog no:895561, R&D Systems, Minneapolis, Minnesota, USA) supplemented with protease inhibitors (Thermo Fisher Scientific

(10) Epithelial-mesenchymal transition induced by SARS-CoV-2 required transcriptional upregulation of SnailAmerican journal of cancer researchJanuary 1, 2021Lai YJ, Chao CH, Liao CC, Lee TA, Hsu JM, Chou WC, Wang J, Huang HC, Chang SJ, Lin YL, et al.

(11) Priming with HDAC Inhibitors Sensitizes Ovarian Cancer Cells to Treatment with Cisplatin and HSP90 InhibitorsInternational journal of molecular sciencesNovember 5, 2020Rodrigues Moita AJ, Bandolik JJ, Hansen FK, Kurz T, Hamacher A, Kassack MU.

(12) Small Extracellular Vesicles Are Key Regulators of Non-cell Autonomous Intercellular Communication in Senescence via the Interferon Protein IFITM3Cell reportsJune 25, 2019Borghesan M, Fafián-Labora J, Eleftheriadou O, Carpintero-Fernández P, Paez-Ribes M, Vizcay-Barrena G, Swisa A, Kolodkin-Gal D, Ximénez-Embún P, Lowe R, et al.

(13) Targeting CREB-binding protein overrides LPS induced radioresistance in non-small cell lung cancer cell linesOncotargetJune 22, 2018Gökyildirim MY, Grandel U, Hattar K, Dahlem G, Schuetz E, Leinberger FH, Eberle F, Sibelius U, Grimminger F, Seeger W, et al.

(14) miR-30e* is overexpressed in prostate cancer and promotes NF-κB-mediated proliferation and tumor growthOncotargetSeptember 15, 2017Egan SM, Karasik E, Ellis L, Gollnick SO.

(15) The release of pro-inflammatory cytokines is mediated via mitogen-activated protein kinases rather than by the inflammasome signalling pathway in keratinocytesClinical and Experimental Pharmacology and PhysiologyJuly 1, 2017Thomas Ondet, Béatrice Muscatelli-Groux, Cédric Coulouarn, Sacha Robert, Thomas Gicquel, Aude Bodin, Vincent Lagente, Jean-Alexis Grimaudpurchased from Cell Signaling Technology (Beverly, MA, USA). Cells were harvested in lysis buffer (ref: 895561, R&D Systems, Minneapolis, USA). The Micro BCA Protein Assay Kit was purchased from Pierce (Rockford,

(16) Induction of Neuroendocrine Differentiation in Prostate Cancer Cells by Dovitinib (TKI-258) and its Therapeutic ImplicationsTranslational oncologyJune 1, 2017Yadav SS, Li J, Stockert JA, Herzog B, O'Connor J, Garzon-Manco L, Parsons R, Tewari AK, Yadav KK.were solubilized in lysis buffer (1×107 cells/mL, R&D Systems with protease inhibitors, catalog number 895561) by gently rocking for 30 minutes at 4°C. Following blocking, 300 μg of proteins were added to the membranes

(17) L1 Cell Adhesion Molecule Promotes Migration and Invasion via JNK Activation in Extrahepatic Cholangiocarcinoma Cells with Activating KRAS MutationMolecules and cellsMay 31, 2017Kim H, Hwang H, Lee H, Hong HJ.

(18) Glycemia and the cardioprotective effects of insulin pre-conditioning in the isolated rat heartCardiovascular diabetologyApril 4, 2017Nakadate Y, Sato H, Oguchi T, Sato T, Kawakami A, Ishiyama T, Matsukawa T, Schricker T.

(19) p63 attenuates epithelial to mesenchymal potential in an experimental prostate cell modelPloS oneJanuary 1, 2013Olsen JR, Oyan AM, Rostad K, Hellem MR, Liu J, Li L, Micklem DR, Haugen H, Lorens JB, Rotter V, et al.fluorescence microscopy. For WB analysis cells were lysed in Lysis Buffer 6 (R&D Systems Inc, Minneapolis, MN, USA, Part# 895561) and Protease Inhibitor Cocktail Set I diluted 1∶100 (Calbiochem, Cat# 535142). Protein concentrations

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells). read more

Lysis Buffer 6 (1 x 21 mL) from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Bench Tips

Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

Lysis Buffer 6 (1 x 21 mL) from R&D Systems, a Bio-Techne Brand

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